World Journal of
Pharmaceutical and Life Sciences

LCMS- A REVIEW

Mohammed Sayeed Uddin*, Dr. Osman Ahmed, Syeda Rakhshinda Zareen, Reshma and Dr. Anas Rasheed

ABSTRACT

Liquid chromatographers often choose LC/MS, a rapidly improving technique that combines LC with mass spectrometry. There is a method called liquid chromatography-mass spectrometry (LC-MS/MS) that combines HPLC (high-performance liquid chromatography) with mass spectrometry. Combining the physical separation skills of high-performance liquid chromatography (HPLC) with the mass analysis capabilities of mass spectrometry, it is an analytical chemistry method. For both qualitative and quantitative examination of pharmacological compounds, drug products, and biological materials, labs often utilise liquid chromatography-mass spectrometry (LC-MS/MS). Metabolic stability screening, metabolite identification, in vivo drug screening, impurity identification, peptide mapping, glycoprotein mapping, natural product de-duplication, and bio-affinity screening are just some of the numerous applications it has found in the pharmaceutical industry. Therapeutic drug monitoring (TDM), clinical and forensic toxicology, and doping control are just few of the fields where LC-MS is being routinely employed with great effectiveness. The demand for more robust analytical and bio-analytical methods that can sensitively and selectively distinguish target analytes from high complexity mixtures has been, and continues to be, a driving force behind the development of LCMS. Because of recent developments in equipment, the combination of liquid chromatography (LC) with mass spectrometry (MS) is now a potent two-dimensional (2D) hyphenated technology.

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