World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

CLONING, EXPRESSION AND PURIFICATION OF THE MANGANESE SUPEROXIDE DISMUTASE (MNSOD) GENE FROM BACILLUS ARYABHATTAI AB211

*Swagata Chakraborty and Dr. Abhrajyoti Ghosh

ABSTRACT

The organism Bacillus aryabhattai AB211,is a plant growth promoting rhizobacteria. It has robust stress responsive genes, which enable them to withstand the oxidative stress condition, generated in the plant rhizosphere. SOD (superoxide dismutase) is basically an enzyme which catalyses dismutation i.e partitioning of the O2-(superoxide radical) into molecular oxygen and hydrogen peroxide. There are mainly three types of SOD genes in Bacillus aryabhattai AB211: FeSOD (which binds Fe), MnSOD( which binds Mn) and Cu/ZnSOD(which binds Cu/Zn). This project mainly deals with the MnSOD genes(located at the cytoplasm). These genes were cloned, to produce the protein and the expression of these proteins were checked at different temperature and at different concentrations of IPTG induction. After expression, purification of the MnSOD protein was done using Ni-NTA Affinity Chromatography, followed by Dialysis and Ion-Exchange Chromatography.

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