World Journal of
Pharmaceutical and Life Sciences

ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITIES OF THE LEAVES OF SRI LANKAN VARIETY OF Durio zibethinus

Hewa Sahabanduge Chani Sahabandu and Srianthie A. Deraniyagala*

ABSTRACT

This study was conducted to determine the total phenolic (TPC) and total flavonoid contents (TFC), antioxidant potential, and anti-inflammatory activity of the aqueous leaves extract of the Sri Lankan variety of Durio zibethinus (Durian) (ALEDZ). The ayurvedic “kasaya” preparation method was used to prepare the extract. The TPC was 90.9±6.5 mg (PGE)/g and the TFC was 77.9±9.2 mg (QE)/g. The ALEDZ displayed the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity (RSA) from 7.9% to 46.5% whereas values for ascorbic acid ranged from 64.3% to 91.5% within the same concentration range (250.0-1500.0 ?g/mL). The ALEDZ showed promising reducing capacity at the concentration range of 25.0-50.0 mg/mL. In the hydroxyl radical scavenging assay, the ALEDZ and ascorbic acid exhibited the RSA between 25.9%-59.8% and 46.9%-77.4%, respectively at the concentration range of 25.0-50.0 mg/mL. The anti-inflammatory activity was determined using two assays. In the NO radical scavenging assay, the plant extract has shown the RSA between 19.8%-55.3%. Within the same concentration range (20.0-100.0 ?g/mL), NO RSA of ascorbic acid varied between 44.1%-78.0%. The human red blood cell membrane stabilization assay showed the protection of 12.2%-51.7% for the ALEDZ and 36.1%-73.1% for aspirin at the concentration range of 200.0-1000.0 ?g/mL. Results of the present study indicate that the aqueous extract of the Sri Lankan variety of Durio zibethinus (Durian) (ALEDZ) has promising antioxidant and anti-inflammatory activities.

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