Abstract
EVALUATION AND VALIDATION OF A UPLC METHOD FOR ESTIMATION OF CAMEL MILK IN MARKETED (ADVIK) DRY POWDER DOSAGE FORM
Dr. Osman Ahmed*, Mohammed Akthar Sulthana, Ashraf Unnisa, Meher Afrin and Dr. Anas Rasheed
ABSTRACT
Following experimental trials that may be summarised, the separation has to be achieved at optimal circumstances. Static phases such as Hypersil BDS C18 were most suited because they provided well-resolved peak shapes with high resolution and excellent sensitivity. Keeping the flow at 1 mL min-1 ensures high resolution. Camel Milk Dry powder was tested for PDA detector response and the wavelength with the maximum sensitivity was determined to be 240 nm. In order to separate Camel Milk Dry powder, a 40:60 percent v/v combination of ethanol and methanol was employed as the mobile phase, moving at 1mL/min. The temperature of the column was kept constant at room temperature.
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