Abstract
CHARACTERIZATION OF FORCE DEGRADATION ASSAY METHOD EVALUATION FOR SIMULTANEOUS ESTIMATION OF ALBENDAZOLE AND IVERMECTIN IN VETERINARY DOSAGE FORM USING UPLC-MS/MSN
Ashraf Unnisa*, Dr. Osman Ahmed, Meher Afrin, Mohammed Akthar Sulthana and Dr. Anas Rasheed
ABSTRACT
In order to achieve the separation under the optimized conditions after experimental trials that can be summarized. Stationary phase like Hypersil BDS C18 (100 mm x 2.1 mm, 1.7 ?m) column was most suitable one, since it produced symmetrical peaks with high resolution and a very good sensitivity and with good resolution. The flow rate was maintained 1.2 mL min-1 shows good resolution. The PDA detector response of Albendazole and Ivermectin, veterinary dosage form was studied and the best wavelength was found to be 215 nm showing highest sensitivity. The developed & validated RP?UPLC-MSMSn methods employed here proved to be specific, fast, precise and accurate for the simultaneous estimation and stability indicating assays as well as related substance quantifications of Albendazole and Ivermectin in combine dosage form.
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