World Journal of
Pharmaceutical and Life Sciences

ISOLATION AND SCREENING OF POTENT ACTINOMYCETES FROM THE SOIL SAMPLES OF SOUTHERN PART OF UTTAR PRADESH, INDIA

Tuba Afsheen*, Sachchidanand, Ajay Kumar Singh

ABSTRACT

Many bacterias are having the potential to produce antibiotics. Among them, actinomycetes are best known for their ability to produce antibiotics. The present study was carried out for the discovery of novel drugs and also new species of actinomycetes which have the potential to produce antimicrobial metabolites. A total of 50 soil samples were collected from different locations of southern part of Uttar Pradesh, India. 12 actinomycetes strains were isolated and screened for their ability to produce antibiotics by spektra-plak method against test organisms. Out of 12 strains, 5 isolates (TAA04, TAA10, TAA12, TAA33 and TAA46) showed potent activity against test organisms. These isolates were further characterized for temperature tolerance and NaCl tolerance tests. Biochemical tests were performed for the identification of the isolates. Based on the findings, isolates were found to be of genus Streptomyces. Further, these isolates were evaluated for antimicrobial activity against test organisms i.e. S. dysenteriae (MCCB-0043), S. aureus (MCCB-0045), K. pneumoniae (MCCB-0026), B. subtilis (MCCB-0222), P. vulgaris (MCCB-R035), P. aeruginosa (MCCB-0034), E. coli (MCCB-0017), and E. feacalis (MCCB-161) by determining the zones of diameter using well diffusion method. Isolate TAA04 showed no zone against P. vulgaris. Isolate TAA12 showed no activity against S. aureus and E. coli. TAA46 was found inactive against S. aureus and P. vulgaris. TAA46 showed highest activity against K. pneumonia, B. subtilis and E. faecalis; TAA10 was highly active against S. aureus and E. coli; TAA04 and TAA12 showed maximum zone for S. dysenteriae and P. vulgaris respectively. The antibacterial activity of the isolates showed potential as a source of antibiotic producers.

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