World Journal of
Pharmaceutical and Life Sciences

HEALING EFFECT OF LEAF EXTRACT OF CANDLEBUSH (CASSIA ALATA L.) ON CUTANEOUS WOUND INFECTED WITH TRICHOPHYTON RUBRUM

Mohammad Kanedi*, Ahmad Rokiban, Subur Widodo, Nopiyansah, Isbiyantoro, Lia Fauziah

ABSTRACT

Research on antimicrobial activity and healing effect of candlebush (Cassia alata L.) extract on wound has been widely done, but its effect on cutaneous wound that were intentionally inoculated with a specific fungus is not known. This study was set to investigate the healing effects of leaf extract of candlebush on rabbit cutaneous wound that intentionally inoculated with T. rubrum. Eighteen male rabbits were divided into six groups: P0,P1, P2, P3 and P4, each consisting of three rabbits. P0 is rabbits that treated with distilled water as the control; P1, P2, P3 and P4 respectively are the rabbits that received candlebush extract of 15%, 25%, 35%, and 45%; P5 is rabbits that were given 2% ketoconazole ointment as the positive control. To inflict wound, the withers area on the animal's back were shaved and the shaved skin was then cut of about 2 cm in lenght using surgical blade. The inflicted wound was inoculated with T. rubrum and bandaged with sterile gauze for five days and after which the bandage is opened the treatment is done topically. Five levels of wound score were used as healing parameters namely: 4 (acute stage), 3, 2, 1, and 0 (recover stage). Leaf extracts of candlebush at the concentration of 45% totally heal the wound on day 21 while the positive control, ketoconazole 2%, gave total healing on the day 18. Thus, candlebush extract is potent to heal cutaneous wound infected by Trichophyton rubrum.

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