World Journal of
Pharmaceutical and Life Sciences

ISOLATION AND CHARACTERIZATION OF NOVEL ACTINOMYCETES STRAINS FROM THE SOIL OF DARJEELING TEA GARDEN

*Swagata Chakraborty and Dr. Maitree Bhattacharyya

ABSTRACT

The organism Actinomycetes was isolated from the soil of the Darjeeling tea garden for carrying out the research. The organism Actinomycetes is a sub-group of Actinobacteria which are Gram- positive and are generally rod-shaped or filamentous in nature. They are generally found in soil and also in the urogenital tract, gums and in the digestive system of humans. The bacteria was grown in starch-caesin media followed by the genomic DNA isolation. PCR was carried out for the amplification of the 16s rRNA gene which is unique in almost all bacterial communities and thus acts as a historical or chronological clock, followed by agarose gel electrophoresis. For bacterial identification Biochemical tests were carried out. The actinomycete strains were further screened for the production of secondary metabolites by cross-streak. Furthur purification was carried out and sequencing was done which was then matched with nucleotide sequences from a number of databases.

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