World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
World Journal of Pharmaceutical and Life Sciences (WJPLS) has indexed with various reputed international bodies like : Google Scholar, Index Copernicus, Scientific Indexing Services (SIS), Indian citation Index (ICI), Directory of Research Journals Indexing, Scintific journal impact factorScientific Journal Impact Factor (SJIF), Jour Informatics (Under Process), UDLedge Science Citation Index, Global Impact Factor (In Process), International Impact Factor Services, International Society for Research Activity (ISRA) Journal Impact Factor (JIF), Science Library Index, Dubai, United Arab Emirates, International Innovative Journal Impact Factor (IIJIF), Scientific Journal Impact Factor (SJIF), Eurasian Scientific Journal Index (ESJI), Cosmos Impact Factor, IFSIJ Measure of Journal Quality, International Scientific Indexing, UAE (ISI), Web of Science Group (Under Process), Research Bible, Fuchu, Tokyo. JAPAN, Scholar Article Journal Index (SAJI), Scope Database, Academia, 

Abstract

PRODUCTION AND PURIFICATION OF APHLA- HEMOLYSIN FROM PROTEUS MIRABILIS PMBS41 FROM URINARY TRACT INFECTION

Essam F. A. Al-Jumaily* and Sara Hussein Zgaer

ABSTRACT

The Proteus mirabilis pathogenic isolates (53 isolates), which isolated for urine samples from patients suffering from urinary tract infection (UTI) were found to produce hemolysin as indicated by greenish zone around colonies grown on blood agar plates. Ten isolates were screened for their production and the highest producing isolate PMBS41 which gave (42.734 Unit/ml) was selected for further experiments. Hemolysin was completely precipitated using 25-50% saturation of ammonium sulphate which gave enzyme activity (128.20unit/ml). Then followed by ion exchange chromatography using DEAE-cellulose (3.5 x 15 cm) gave only one of the many peaks showed hemolytic activity gave (48.42 unit/ml) and a yield (45.42 %), then gel filtration using Sepharose 6B column (1.5 x85 cm) gave (42.24 unit/ml) and a yield (14.90 %).

[Full Text Article]   [Download Certificate]

WJPLS CITATION

  All Since 2019
 Citation  422  322
 h-index  9  7
 i10-index  4  2

SJIF Impact Factor 6.12  

INDEXING

Indexing

NEWS & UPDATION

    A PHP Error was encountered

    Severity: Notice

    Message: Undefined variable: news

    Filename: views/right_panel.php

    Line Number: 79

    A PHP Error was encountered

    Severity: Warning

    Message: Invalid argument supplied for foreach()

    Filename: views/right_panel.php

    Line Number: 79

BEST ARTICLE AWARDS

World Journal of Pharmaceutical and life sciences is giving Best Article Award in every Issue for Best Article and Issue Certificate of Appreciation to the Authors to promote research activity of scholar.

Best Article of current issue

Download Article : Click here

Downloads

  Covering Letter

  Copy Right Form

  Author Instruction

read more

Powered By WJPLS ! All Rights Reserved.

WJPLS

Follow us on: