World Journal of
Pharmaceutical and Life Sciences

PHYTOCHEMICAL EVALUATION AND FLUORESCENCE ANALYSIS OF SOLANUM NIGRUM L.

H. Deepa and Dr. A. Subhashini*

ABSTRACT

Solanum nigrum L. belongs to the Solanaceace family has been used in traditional folk medicine. It has effects of antipyretic, diuretic, anticancer, anti-ulcer and hepatoprotective and therefore it has important medicinal values. Objective: The present study was aimed to investigate the preliminary phytochemical screening and fluorescence analysis of stem, leaf and unripened fruits of Solanum nigrum L. This study represents a report of qualitative determination of phytochemicals of Solanum nigrum L. Methods: The phytochemical constituents like Alkaloids, Saponins, Tanins, Flavonoids, Carbohydrates and Proteins were investigated using Aqueous, Ethanol, Acetone and Chloroform extracts qualitatively. The fluorescence character of the plant sample was studied both in daylight and UV light after treatment with different reagents like methanol, petroleum ether, sodium hydroxide, hydrochloric acid, sulphuric acid, nitric acid and ferric chloride. Results: The qualitative phytochemical studies have clearly demonstrated that the plant Solanum nigrum L is a rich source of alkaloids, flavonoids, saponins, phenols, carbohydrates and protein. Aqueous extract of stem, leaves and unripened fruit contained more phytochemical constituents. The. Furthermore, Flourescence analysis of stem, leaves and unripened fruits confirmed the strandardization of quality, purity of the sample. Conclusion: Among the extracts, the aqueous extract has found to be rich in phyto constituents, which gave a strong reason to select this plant for pharmacological studies.

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