World Journal of
Pharmaceutical and Life Sciences

MORPHOLOGICAL ASSESSMENT OF HUMAN EMBRYOS AND EMBRYO CULTURE USING EMBRYO SCOPE

Dr. VDS Jamwal, Dr. Mohan Angadi*, Dr. Sushil Kumar and Dr. B K Mishra

ABSTRACT

Human embryos show a considerable degree of plasticity and can be cultured in diverse set of culture conditions. Embryos are traditionally cultured in a CO2 incubator which maintains a fairly constant temperature (370C) CO2 levels (6-7%) to maintain a pH of 7.3-7.4 in the culture medium. Due to frequent door opening of the incubators during the morphologic assessment of embryos and the resultant fall in temperature and pH of the culture medium which significantly affects the embryo metabolism, a need was felt for a non invasive in-built dynamic monitoring system for the embryo development.The development of the Trigas bench top incubators enables direct heat transfer to the culture dish and a direct purging of the gas mixture (79% N2,6%CO2and 5%O2)to maintain constant temperature and pH of the embryo culture medium.

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