World Journal of
Pharmaceutical and Life Sciences

ANIMAL MODEL ASSESSMENT OF THE BIOCHEMICAL EFFECT OF ADMINISTRATION OF AQUEOUS EXTRACTS OF A. INDICA STEM BARK

Ihimire Inegbenose Godwin*, Odia Amraibure and Onobun Desmond Odiamehi

ABSTRACT

This study investigated the biochemical implication of administration of aqueous extracts of Azadirchta indica stem bark in animal model. Eighteen (18) Albino rats of first filial generation were used randomly divided into six groups of three rats each based on their body weight. Group A served as the control while group B which served as the safe dose group received 547.23mg/kg body weight in 1mL of extract. In the same manner, group C, D, E and F respectively received 1mL of extract that were 10% below safe dose, 10% above safe dose, 20% above safe dose and 30% above safe dose respectively. Administration was for fourteen (14) days. On the fifteenth (15th) day, all animals were humanely sacrificed and appropriate tissues for analysis were used to assess Lactate dehydrogenase (LDH), Glutathione peroxidase (GPx) or reductase (GSH), Malondialdehyde (MDA), Gamma glutamyltransferase (GGT) among others. Mean values of triplicate analysis were subjected to single analysis of variance and Turkey krammer multiple comparison post hoc test using GraphPad Prism version 7. Results were considered significant at 95% confidence. Group A recorded comparable G-6-PDH activity as group B, 167.21±1.98. Group B recorded the highest GPx activity. GGT observed in group B, 2.32±0.67U/L was significantly (P< 0.05) lower than observed in group A, 6.18±1.39U/L. Administration of the extract resulted in increased LDH activity. Evidence from the study suggest5 beneficial effect of usage of adequate dosage of the extract.

[Full Text Article]   [Download Certificate]