World Journal of
Pharmaceutical and Life Sciences

BIOREMEDIATION OF NICKEL CONTAMINATED SOIL USING BACTERIA

Abhishek Kumar Pandey* and Abhilasha Shrivastava

ABSTRACT

Bioremediation is the use of living organisms (primarily microorganisms) for removal of a pollutant from the biosphere. It relies on biological processes to minimize an unwanted environment impact of the pollutants. The microorganisms in particular have the abilities to degrade, detoxify and even accumulate the harmful organic as well as inorganic compounds. Soil samples were collected from J P Cement Plant, Rewa from different places at a depth of 6 -10 Inches from 4 - 6 different spots. These soil samples were mixed properly and enriched for Nickel resistant clones by incubating 10g of slag in 90 ml of sterile water amended with 10 ml Luria Bertani (LB) medium and 20 ?g/ml each of nickel sulphate at 37°C for 2h. Supernatants were plated at 10-2 dilution by spread-plate method on LB agar medium. The plates were then incubated at 37°C. The colonies appeared after 3 days. Strains were preserved and phenotypic studies were carried out. The metal accumulation efficiency was measured by Atomic Absorption Spectroscopy (AAS). The soil samples collected are all alkaline in nature, the isolated bacteria are gram positive, rod shaped, aerobic, salt tolerant, endospore forming bacteria and according to this research sample with bacterial inoculation in them shown reduction in the Nickel levels as compared to the raw soil samples.

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