World Journal of
Pharmaceutical and Life Sciences

ANTAGONISTIC EFFECT OF LACTOBACILLUS SPECIES ISOLATED FROM DAIRY PRODUCTS AND FERMENTED FOOD ON INTESTINAL PATHOGENIC BACTERIAL SPECIES

*Neha Dubey and Shobha Shrivastava

ABSTRACT

The probiotic microorganisms especially Lactic acid bacteria (LAB) are reported to produce many metabolites including organic acids, hydrogen peroxide, diacetyl and bacteriocins; that could be utilized in vast number of applications. Besides the use of probiotic organism in variety of industrial food and feed fermentations, they possess strong antagonistic properties towards other related microorganisms, including food spoilage organisms and pathogens. Since microbial metabolites derived from probiotics have shown the inhibitory activity against the many pathogens including E.coli, S. aureus, S. typhi, S. dysenteriae, B. anthracis etc. In present investigation also different types of fermentating food stuffs and dairy products were used to isolate probiotic Lactobacilli with antimicrobial potential which probably may secret antimicrobial proteinaceus substances which are collectively called as bacteriocins. The pure culture of indigenously isolated Lactobacilli were inoculated in sterile MRS broth and incubated at 37°C for 48 hours. The Lactobacillus isolates were cultured in the volume of 5 ml in test tube which were subjected to centrifugation at 10,000 RPM in a high speed centrifuge for 15 minutes at ambient temperature after the period of incubation when growth of microbes were observed in the form of turbidity. The procedure followed was with reference to the procedures suggested. Well diffusion method was adapted to check the antimicrobial potential of indigenously isolated Lactobacilli from different fermenting food and dairy products Out of the 14 indigenously isolated Lactobacillus spp. the isolates which were observed to impart inhibitory effect at a concentration of 20 ?l of fermentation extract in each well of media plates are LB-2, LB-5, LB-6, LB-7, LB-11, LB-12, LB-13 and LB-14 towards test microbial species used in present work.

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