World Journal of
Pharmaceutical and Life Sciences

AN IN VITRO STUDY OF IPOMEA CARNEA AND PIPER BETEL LEAF EXTRACT MIXTURE ON ANTIDIABETIC AND BACTERIAL ENDOTOXIN NEUTRALIZATION

Saranya S.*, Ramachandran A. M., Manju M. and Harsha Karunakaran

ABSTRACT

The main objective of this study was to analyse the rate of glucose transport across cell membrane in yeast cell system in the presence of the ethanolic extract of Ipomoea carneaand Piper betel leaves and to neutralize the bacterial endotoxin which causes wounds to the diabetic patient. Ipomoea carnea and Piper betel leaves has anti diabetic property and collected from kurumbapalayam. The ethanolic extract of leaves was prepared to analyse the phytochemical and antidiabetic activity done with the yeast cell system method. The lipopolysaccharide (LPS) from E.coli was separated using chloroform-methanol solvents. The LPS was treated with the leaf extract and monitored the neutralization of the E.coli toxin by SDS - PAGE method. The amount of glucose uptake was high in mixture of both leaf extract. The leaf extract of both Ipomoea carnea and Piper betel mixture was neutralized the LPS of E.coli. From this study we can reduce the adverse effect of allopathic medications and also acquire a permanentrecovery.

[Full Text Article]   [Download Certificate]