World Journal of
Pharmaceutical and Life Sciences

MOLECULAR PHYLOGENETICS OF SELECTED ADIANTUM SPECIES USING RBCL SEQUENCING STUDIES

A. Antony Selvi, S. Christopher Patrick Kiladi and T. Leon Stephan Raj*

ABSTRACT

Plants are used as a source of medicine in Traditional Medicinal Systems in India from time immemorial. The demand in medicinal plants leads to the adulteration in pharmaceutical industries. This study was performed to identify and analyze the phylogenetic relationship among four species of Adiantum, A. latifolium, A. incisum, A. hispidulum and A.caudatum using rbcL sequencing studies. The rbcL sequencing of the selected plants analysed with BLAST for similar sequence studies and the alignment was done with T-Coffee software. BLAST results indicated that the number of similar sequences present in the Genbank repository. The phylogenetic tree represented the degree of similarity andvariation with other species and other accessions of same species. Comparative analyses of these DNA barcode sequences revealed species-specific nucleotide sequences capable of discriminating the four Adiantum species. Phylogenetic analyses also revealed four distinct clusters showing homogeneous clades with high resolution at the species level. The result of the study was useful for the authentication ofselected adiantaceae members. The phylogenetic tree showed their relationship with other adiantaceae members.

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