World Journal of
Pharmaceutical and Life Sciences

ISOLATION AND IDENTIFICATION OF LACTIC ACID BACTERIA FROM PAMPANGAN BUFFALO MILK OF SOUTH SUMATERA INDONESIA

Heni Rizqiati*, Nurwantoro, Sri Mulyani and Andi Febrisiantosa

ABSTRACT

Pampangan buffalo milk is one of lactic acid bacteria (LAB) source which potentially can be used as starter or probiotic starter. The objective of this research was to isolate and identify LAB derived from Pampangan buffalo, South Sumatera. Lactic acid bacteria was isolated and grown at De Man Rogosa Sharpe Agar (MRSA). Isolation was performed until pure culture was obtained. LAB identification was carried out through morphology, physiology and biochemical characteristic test. Morphological test was done through Gram staining and cell shape identification. Physiological characteristic was done through survivability test on pH 4.5 and temperature 45 oC. Biochemical characteristic was carried out through catalase test, identifying the production of CO2, NH3, and dextran. A total of 21 LAB isolates were identified which generally known having Gram positive, rod and rounded shape. From the result of physiological and biochemical test, 10 LAB isolates from Pampangan buffalo milk were found i.e. SKP2, SKP3, SKP5, SKP6, SKP9, SKP10, SKP14, SKP19, SKP20 and SKP21 isolates. API test CHL 50 test resulted that five LAB species were found from 10 selected isolates contained in Pampangan buffalo milk. Those LAB species were L. brevis, L. paracasei, L. pentosus, L. plantarum and Lactococus lactis.

[Full Text Article]   [Download Certificate]