World Journal of
Pharmaceutical and Life Sciences

PRELIMINARY ANALYSIS OF PUMPKIN SEED EXTRACT FOR PHYTOCHEMICAL, ANTIOXIDANT ABILITY, CYTO-TOXICITY AND COLLAGEN SYNTHESIS

Akashraj R. Agrahari, Pranjali P. Dhawal and Dr. Tanuja V. Tirodkar*

ABSTRACT

Pumpkin seed extracts are greatly studied and have gained importance due to its nutritional benefits. The present study aims to explore other properties beneficial to mankind. The crude extract of pumpkin seed was obtained by cold extraction method. This crude extract was further analyzed for anti-oxidant activity using 2,2-Diphenyl picryl hydrazyl method (DPPH method) . The Phytochemicals present in the seed extract were found by the preliminary methods. Cyto-toxicity analysis on 3T3/NIH Cell line was carried using MTT assay. The Collagen synthesis activity of the crude extract was also tested on 3T3 cell line. After testing all the parameters of the Pumpkin seed crude extract, it was found that it had DPPH radical scavenging ability. The extract when tested for toxic effects showed that even at concentration 1 mg/mlit had proliferative ability as compared to cell with media and without extract. The extract also boosted the Collagen synthesis by 3.91 pg/ml as compared to media control. The properties exhibited by Pumpkin seed crude extract could be recommended in Pharmacological and Cosmetological applications with proper clinical trials.

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