World Journal of
Pharmaceutical and Life Sciences

IN VITRO SELECTION Phalaenopsis amabilis (L.) Bl. PLANTLETS RESULT OF INDUCED RESISTANCE WITH FUSARIC ACID

Endang Nurcahyani*, Sumardi, Hardoko Insan Qudus, Sri Wahyuningsih, Sholekhah and Asma Palupi

ABSTRACT

Phalaenopsis amabilis (L.) Bl. is an original orchid from Indonesia and one of Indonesia's national flowers, known as "Puspa Pesona", is included in the list of endangered species. P. amabilis is also one of the most popular orchid plants for various groups of people because of the beauty of the shape and color of flowers, but the production of P. amabilis in Indonesia is still far behind compared to other countries such as Thailand, Taiwan, Singapore and Australia. Fusarium wilt is caused by Fusarium oxysporum (Fo) which until now has not been able to be overcome effectively. The use of P. amabilis plantlets that are resistant to Fusarium wilt is expected to be an important alternative for disease control. The purpose of this reserach was to study and determine the concentration of Fusaric Acid (FA) in the selection of P. amabilis plantlets that were tolerant of Fusarium wilt. This study used P. amabilis plantlets with 5 levels of FA concentration, namely 0 ppm, 10 ppm, 20 ppm, 30 ppm, and 40 ppm. The results showed that the concentration of FA tolerant for optimum growth was 40 ppm. The results of in vitro selection with subcultured FA on multiplication medium resulted in a number of live P. amabilis plantlets is 100% (10 -30 ppm), which were insensitive to FA, whereas at 40 ppm a number of live P. amabilis plantlets is 60%.

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