World Journal of
Pharmaceutical and Life Sciences

IN VITRO ASSESSMENT OF GARLIC EXTRACT AND PURIFIED ALLICIN AGAINST LIVER (HEPG2) AND COLORECTAL (HCT-116) CANCER CELL LINES

Amany Mohamed Khalil, Soheir Saad Abd El Salam*, Tamer M.M.Abuamara Mervat G. Hassan, Aly Fahmy Mohamed, Dr Maha Anwer and Hala Abd El Aziz

ABSTRACT

Cancer is one of the major public burden worldwide. It is a multicellular disease that can arise from all cell type. In the recent decades, the number of cancer related showed a clear elevation, in turn creating huge health and economic problems. Garlic has been used as folk medicine approach for over 4000 years, and was the most widely researched medicinal plant. The present study showed that garlic extract and Allicin can induce inflammatory responses, inducing apoptosis. Liver and colorectal cancer cell lines were tested for the anti-cancer activity of test materials regarding the up-regulation of pro-apoptotic gene, Bax and P53, also anti-apoptosis gene, Bcl-2 down regulation. Toxicity of garlic extract and Allicin to HCT-116 was cell type and concentration dependent and the IC50 value recorded was 4.16?g/ml for Allicin and 16.6?g/ml for garlic extract. While it was 48 ?g and 2.49?g/ml for HEPG-2 using garlic and Allicin it was respectively. Regarding the anticancer potential it was noted that pro and anti-apoptotic genes showed a remarked up and down regulation respectively compared to the genetic profile of untreated cell control. On the genetic level anti-apoptotic gene Bcl-2 and pro-apoptotic gen Bax and p53 were tested to prove the postulation that the garlic extract and Allicin can be used in dealing with cancer cells. In the meantime cellular DNA distribution showed cell arrest during the G2/ M phase of cell division.

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