World Journal of
Pharmaceutical and Life Sciences

DEVELOPMENT OF NANO-MATERIALS FOR THE IMMOBILIZATION OF BIOMOLECULES

Hammad Shuaib*, Mohammed Abdul Jabbar and Munazza Tahreem

ABSTRACT

Nanotechnology is a fast growing field with great promises for providing us many breakthrough that will change the direction of technological advances in a wide range of application. There are two general approaches for the synthesis of different nanoparticles and development of nanostructures. These approaches are: top-down or bottom-up strategies which open up novel avenues for myriads of applications. Bottom-up fabrication methods include the miniaturization of material components to the atomic scale with further self-assembly process leading to the construction of nanostructure. In the present work, the urea sensing film formed by encapsulated enzymes show potentiometric response of the modified gold electrode (Urs/Alginate/Nano-Au) that depends on the mass transportation of the substrate (urea) to the mod+ified workin?g electrode containing encapsulated urease and forming electro-active species

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