World Journal of
Pharmaceutical and Life Sciences

HEPCIDIN DOWNREGULATION THROUGH INHIBITION INTERLEUKIN-6 RECEPTOR AND JAK/STAT3 SIGNALING WITH EXTRACT OF PHYSALIS MINIMA

Rudy Salam* and Diana Lyrawati

ABSTRACT

Inflammation have an impact on iron homeostasis through to activation of hepcidin. Hepcidin is a peptide with 25 amino acids that are expressed mainly in the liver and iron-regulatory molecule. Inflammation stimulates hepcidin expression and causes limitation of iron availability. The inflammatory cytokines, especially IL-6, activate hepcidin through activation of STAT3-mediated signaling to absorb iron in macrophages. The goal of this study was to investigate the inhibition effect of an ethanolic extract from the plant Physalis minima L. (Solanacae) on hepcidin expression in zebrafish. In this study, zebrafish were divided into 5 groups: negative control, positive control (exposed with LPS), treatment group 1 (exposed with LPS and 1% extract), treatment group 2 (exposed with LPS and 2,5% extract) and treatment group 3 (exposed with LPS and 5% extract). Results from this experiment indicated that hepcidin expression significantly decrease on treatment group compared with positive groups but there is no significant difference between control groups and treatments groups on IL-6 and STAT3 expression. It could be concluded that an ethanolic extract from the plant Physalis minima L. (Solanacae) could decrease the expression of hepcidin but not IL-6 and STAT3.

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