World Journal of
Pharmaceutical and Life Sciences

ISOLATION, PARTIAL CHARACTERIZATION OF A FLAVONE FROM SUDANESE PULCARIA CRISPA STEMS AND BIOLOGICAL ACTIVITY OF DIFFERENT FRACTIONS

Abdel Karim M.*, Lubna A. and Thohami E. Hagr

ABSTRACT

Pulicaria crispa is used in folk medicine for the treatment of colds, coughs, colic pain, excessive sweating and as carminative. Phytochemical screening of pulcaria crispa stems revealed the presence of flavonoids. A flavonoid - compound I- was isolated from the ethanol extract by column and paper chromatography and its structure was partially characterized on the basis of its spectral data (IR, UV and NMR). The crude extract and different fractions (chloroform, n- butanol and ethyl acetate) of pulcari crispa were screened for their antimicrobial activity against five standard human pathogens. The ethyl acetate fraction of pulcaria crispa showed moderate activity against Staphylococcus aureus, Escherichia coli and Candida albicans. However, the ethanol extract and chloroform fraction were inactive against the tested bacteria, but they gave partial antifungal activity against the fungus Candida albicans. The n-butanol fraction exhibited partial activity against Staphylococcus aureus and Candida albicans.

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