World Journal of
Pharmaceutical and Life Sciences

PURIFICATION OF ANTIMICROBIAL COMPOUND FROM ACTINOMYCES SP AGAINST SALMONELLA TYPHIMURIUM

Khin Swe Mya*, Thein Soe, Mu Mu Aung Soe, Thida Soe, Lei Lei Oo and Myat Thandar Swe

ABSTRACT

In the study, isolation of microorganisms was done by dilution method. The strain was isolated from Turritella carinifera. The strains showed the antibacterial activity against Salmonella typhimurium. The results of the biochemical tests reveal that the strain was Actinomyces species. According to the results of paper chromatography (bioautography), it was considered that ethyl acetate was the best solvent to extract the bioactive compound from the fermented broth. Based on the results of thin layer chromatography (TLC), silica gel chromatography was undertaken. The fractions were collected and tested for the antibacterial activity against Salmonella typhimurium. The purified fractions of hexane from re-chromatography were collected and tested for the antibacterial activity. The purified and active fractions were collected and the biological activity was investigated. The results showed that 17.6 mg of purified bioactive compound could be isolated from 14 liters of fermented broth of Actinomyces species.

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