World Journal of
Pharmaceutical and Life Sciences

DEEP SEA Bacillus flexus AND THE EVALUATION OF ANTI-MYCOBACTERIAL ACTIVITY

Tutik Murniasih*, Regine Citra, Masteria Yunovilsa Putra, Ratih Pangestuti and Endang Sukara

ABSTRACT

Pathogenic bacteria, especially Mycobacterium, happens to be one of the cause of disease in society. The fact that this bacterium is resistant to several commercial antibiotics, meanwhile the development of new effective antibacterial compound to overcome this problem is needed. The search of antibacterial compound in this research was done with the source from deep sea. The aims of the study was to find the new source of antimycobacterium producers as well as to characterize the potential active compound. The selected deep-sea microorganism was cultured aerobically under room condition (29oC, 1 ATM). The cultivation under room condition was done using 10x 1.5 L M2 medium broth. Antibacterial compound was extracted using ethyl acetate, and separated using HPLC. Preliminary active compound identification was done using GC-MS. The antibacterial activity of each fraction was examined. The result from GC-MS showed that the suggested active compound was 1,2-benzenedicarboxylic acid, mono (2-ethylhexyl) ester which showed moderate activity against Mycobacterium smegmatis. Molecular characterization of potential strain showed 99% similarity with Bacillus flexus. Furthermore, in this study proved that this deep sea B. flexus could grow without adjusting to the extreme condition such aslow temperature, high pressure and low lighting.

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