World Journal of
Pharmaceutical and Life Sciences

A UV SPECTROPHOTOMETRIC ASSAY OF SUSTAINED RELEASE BRANDS OF TRAMADOL HYDROCHLORIDE

S. Naga Gayatri and Dr. V. M. Biju*

ABSTRACT

Tramadol hydrochloride is an analgesic used to treat moderate to moderately severe pain. A simple efficient least time consuming spectrophotometric technique for the assay of tramadol hydrochloride has been developed. The Assay is based on Ultraviolet UV absorbance maxima at about 228 nm wavelength of tramadol hydrochloride using water as solvent. A sample of drug was dissolved in water to produce a solution containing 200 ppm tramadol Hydrochloride. Similarly a Sample of different brands were dissolved in water to produce a solution containing 200 ppm solution. The absorbance of sample preparation was measured at 228 nm against the solvent blank. Samples were prepared in the range of 200-12.5 ppm concentration for two different brands. Their % Assay, Regression Equation, Regression line, correlation co-efficient is obtained to predict further availability of drug. Thus we can conclude that the method can be applied for the routine Quality control quantitative analysis of tramadol hydrochloride sustained release formulation.

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