World Journal of
Pharmaceutical and Life Sciences

EFFECTS OF EXTRINSIC FACTORS ON ASYMBIOTIC GERMINATION OF THREATENED ENDEMIC MALAGASY ORCHIDS: EULOPHIELLA ROEMPLERIANA, GRAMMANGIS ELLISII AND GRAMMANGIS SPECTABILIS

Rahelivololona Raharitiana and *Ravoniarison Nivohanintsoa Elinorovololona

ABSTRACT

The endemic Orchids of Madagascar are threatened with extinction because of the deforestation and their excessive collect. It?s urgent to search the means of reproduction and the preservation in order to limit this danger. The control of the technics appropriated to the malagasy orchids especially for the species Eulophiella roempleriana, Grammangis ellisii and Grammangis spectabilis, was the main objective of this investigations. In vitro seeds germination of orchids depends on intrinsic factors related to the quality, viability, maturity of seed and on extrinsic factors such as physical and chemical conditions: temperature, light, culture medium. In order to obtain high rate of germination, some factors have been assessed successively. The interaction of pretreatment 26°C during 10 days followed by the treatment of 15 days in the dark condition, has improved the speed and capacity of germination of Eulophiella roempleriana, Grammangis ellisii and Grammangis spectabilis. While pretreatment 7°C delay the germination of these three species. The three basis media of MS/2 and those of Knudson, Vacin and Went each added with vitamin proved to be suitable for the germination of these 3 species. The sucrose failure in the medium inhibits their germination. The sucrose concentration to 30g/l has given a best result.

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