World Journal of
Pharmaceutical and Life Sciences

STUDIES ON DEVELOPMENT OF FIBRINOLYTIC ENZYME STREPTOKINASE ISOLATED FROM BACILLUS SUBTILIS

Dr. P. Lakshmaiah*, Sk. Faruk, K. Hemanth Venkata Manisai, V. Siva Prasad, K. Sai sowmya and U. Spandana

ABSTRACT

Streptokinase is an extracellular protein, novel fibrinolytic enzymes which are isolated from Bacillus subtilis,it is a non-protease plasminogen activator that activates plasminogen to plasmin, the enzyme that degrades fibrin cloth through its specific lysine binding site; it is used therefore as a drug in thrombolytic therapy. The rate of bacterial growth and streptokinase production was studied in condition of excess glucose addition to culture media and its pH maintenance. The streptokinase product of the bacterial culture was preliminary extracted by salt precipitation and then purified by affinity chromatography on plasminogen substituted sepharose-4B in a condition that the plasminogen active site was protected from streptokinase-induced activation. The purity of streptokinase was confirmed by SDS-PAGE and its biological activity determined in a specific streptokinase assay. The results showed that in the fed–batch culture, the rate of streptokinase production increased over two times as compared with the batch culture while at the same time, shortening the streptokinase purification to a single step increase.

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