World Journal of
Pharmaceutical and Life Sciences

ISOLATION, PRODUCTION AND EXTRACTION OF BACTERIAL POLYHYDROXYALKANOATE ENZYME

Hafiz Khawar and Sikander Ali*

ABSTRACT

Polyhydroxyalkanoate is an enzyme. It is produced from microorganisms and plants. The most efficient production of PHA enzyme through microorganism. Because, it is naturally produced by bacterial species. It is also a subclass of polyhydroxybutyrate. PHA is a polyester compound and have the ability of biodegradable property. It is also biocompatible compound. PHA present in bacteria as inclusion bodies. The Sudan B Black stain used to screen the inclusion body of bacteria under 100x microscope. Phasins protein present in lipid membrane of bacteria that provide the stability and biosynthesis of PHA. PHA is monomeric compound and it help to increase the identification of naturally occurring PHA through physical and chemical properties. PHA is hydrophobic in nature and in crystal form. A number of bacterial include for the production of PHA such as Protomonas extorguens, P. aeruginosa, P. putida, P. oleovorans, Ralstonia eutropha, E. coli, Bacillus cereus, Bacillus subtilis. Substrate and polyester synthase formation spherical form of granules in the bacterial cells. The in-vitro production of PHA through the cell by using precursor substrate to synthesis of PHA. The In-vivo production of PHA from the cell by the action of amphipathic PHA synthase to synthesis PHA granules. The recovery of PHA from bacterial cells by using different techniques such as chemical, mechanical and enzymatic digestion to isolate and extraction of PHA enzyme. PHA has big advantage to use in industrial and medical application. The use of recombinant strain could be more effective because of renewable carbon sources and cheap. At industrial level, PHA replace synthetic plastic by degrade plastic and also for waste water treatment. In medical application, drug delivery system has great efficient to target area by coating of PHA with drug. The economical cost of PHA is reduce as to synthetic polyester materials.

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