World Journal of
Pharmaceutical and Life Sciences

OPTIMIZATION OF CULTURE CONDITIONS OF SOME THERMOPHILIC FUNGI FOR SELECTION OF A POTENTIAL THERMOPHILIC XYLANOLYTIC STRAIN FROM RAIPUR

Preeti Singh Parihar* and Vibhuti Rai

ABSTRACT

The objectives of the present study were optimization of culture conditions for xylanase production from some thermophilic fungal isolates. A total of 55 fungal isolates were isolated and screened for xylanase activity. Five isolates with higher xylanase activity, higher mycelial biomass at 45oC and lowest percent protein coagulation at 50oC were selected as thermophlic fungi. Xylanase production in five fungal isolates namely Chrysosporium tropicum NFCCI2531, Malbranchea cinnamomea MTCC11894, Aspergillus fumigatus NFCCI2532, Aspergillus terrus NFCCI2533 and Emericella nidulans NFCCI2538 were compared and optimized using different cultural parameters including effect of cheaper raw substrates, different carbon and nitrogen sources, different pH and temperatures. All optimization studies indicated extraordinary capabilities of M. cinnamomea MTCC11894 for production of extracellular xylanase in presence of wheat bran as raw substrate, fructose and yeast extract as carbon and nitrogen sources, at pH 6.5 and temperature 45°C to 50oC. Thus, present study originates a thermophlic fungi having capability of growing up to 50oC and so can be used for producing thermotolerant xylanase for industrial applications.

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