World Journal of
Pharmaceutical and Life Sciences

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
World Journal of Pharmaceutical and Life Sciences (WJPLS) has indexed with various reputed international bodies like : Google Scholar, Index Copernicus, Indian Science Publications, SOCOLAR, China, Cosmos Impact Factor, Research Bible, Fuchu, Tokyo. JAPAN, Scientific Indexing Services (SIS), Jour Informatics (Under Process), UDLedge Science Citation Index, Global Impact Factor (In Process), International Impact Factor Services, International Scientific Indexing, UAE, International Society for Research Activity (ISRA) Journal Impact Factor (JIF), Science Library Index, Dubai, United Arab Emirates, International Innovative Journal Impact Factor (IIJIF), Scientific Journal Impact Factor (SJIF), Eurasian Scientific Journal Index (ESJI), Indian citation Index (ICI), IFSIJ Measure of Journal Quality, International Scientific Indexing, UAE (ISI), 

Abstract

SIMULTANEOUS ESTIMATION OF METFORMIN AND PIOGLITAZONE BY RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF BULK AND PHARMACEUTICAL DOSAGEFORM

S. Srinivasa Rao*, Rathnakar Nathi, B.Anusha, B.Saroja, B.Shrija, K.Manisha

ABSTRACT

A specific, accurate, precise and reproducible HPLC method has been developed and subsequently validated for Metformin and Pioglitazone in commercial tablets. The proposed HPLC method utilizes Agilent eclipse XDB C18 column (150 mm - 4.6 mm i.e., 5 ?m) and mobile phase consisting of Acetonitrile: NaH2PO4 pH 5.00 (50:50 v/v) at a flow rate of 1.0 mL/min. Quantitation was achieved with UV detection at 232 nm and 246 nm based on peak area with linear calibration curves at concentration range 50-800 ?g/mL and 7.5-120 ?g/mL for Metformin and Pioglitazone (R2> 0.999 and 1.0). The method was validated in terms of accuracy (% recovery 99.6% and 99.5), precision (%RSD 0.04 and 0.05), linearity, limits of detection (4.1 ng/ml), limits of quantitation (12.6ng/ml), assay (100.5%), and robustness. This method has been successively applied to pharmaceutical formulation.

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