World Journal of
Pharmaceutical and Life Sciences

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
World Journal of Pharmaceutical and Life Sciences (WJPLS) has indexed with various reputed international bodies like : Google Scholar, Index Copernicus, Indian Science Publications, SOCOLAR, China, Cosmos Impact Factor, Research Bible, Fuchu, Tokyo. JAPAN, Scientific Indexing Services (SIS), Jour Informatics (Under Process), UDLedge Science Citation Index, Global Impact Factor (In Process), International Impact Factor Services, International Scientific Indexing, UAE, International Society for Research Activity (ISRA) Journal Impact Factor (JIF), Science Library Index, Dubai, United Arab Emirates, International Innovative Journal Impact Factor (IIJIF), Scientific Journal Impact Factor (SJIF), Eurasian Scientific Journal Index (ESJI), Indian citation Index (ICI), 

Abstract

HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF AMLODIPINE AND ATENOLOL AND PHARMACEUTICAL DOSAGE FORM

*Shaik Munwar, Y. Bala Akhil, T. Subramaneswara Rao, T. Gopinadh, M. Hemanth Kumar

ABSTRACT

A Simple, specific and sensitive an isocratic Estimation by RP-HPLC analytical Method were developed and validated for the quantification Amlodipine. And Atenolol Quantification was achieved by by using the mobile phase (55 volumes of mixed phosphate buffers ph-4.0: 45 volumes of acetonitrate sonicate for 10 mins for removing gases). Inertsil BDS C18 250×4.6mm ID, 5?m Particle size was used as stationary phase. The flow rate was 1.0 ml/min. Measurements were made at a isobestic wavelength of 220nm. The average retention times for Atenolol And Amlodipine was found to be 6.060 & 2.593 min. The proposed method was validated for selectivity, precision, linearity and accuracy. The assay methods were found to be linear Atenolol And Amlodipine from 60-140?g/ml & 6-14 ?g/ml for respectively. All validation parameters were within the acceptable range. The developed methods were successfully applied to estimate the amount of Atenolol And Amlodipine.

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