World Journal of
Pharmaceutical and Life Sciences

PRODUCTION AND CHARACTERIZATION OF AMYLASE FROM ASPERGILLUS NIGER

Dr. P. N. Rajarajan*, K. Rajeswari, Dr. P. Jeganathan, N. Sumathy, Dr. S. Krishnamoorthy, Dr. G. Chandran

ABSTRACT

The fungal isolates from soil were screened for amylase production and the isolates identified as Aspergillus niger. The isolated organism was cultivated in PDA medium & spread plate techniques were used for isolation. The morphological character was identified using lacto phenol cotton blue stain. Strains were maintained in PDA slants and it is subcultured for every weeks. Screening procedure is carried out to identify the amylase production by Aspergillus niger. The mycelia matt from the production medium is obtained after incubation period and subjected for centrifugation. The crude enzyme obtained from the supernatant is used for further analysis and enzyme activity. Based on the results obtained the enzyme activity of amylase by Aspergillus niger were found to be 400 units/ml. Based on optimum pH, temperature, incubation period, carbon source and nitrogen source. The amylase production were maximum at pH 5(415 U/ml), temperature 30oC (400 U/ml), incubation period 120 hours (5th days) 400 U/ml, Starch 350U/ml, peptone 330 U/ml respectively. On the light of the obtained results, it could be conclude that both nutritional and cultural conditions were required for optimum growth and production of amylase from Aspergillus niger. Aspergillus niger isolated from soil can be industrially exploited for the synthesis of amylase and strain improvement studies can be carried out to enhance enzyme production.

[Full Text Article]   [Download Certificate]