World Journal of
Pharmaceutical and Life Sciences

BIOPROSPECTING OF ENDOPHYTIC ACTINOMYCETES FROM PLUMBAGO ZEYLANICA (LINN.) FOR ANTIFUNGAL ACTIVITY

Sakshi Gandotra*, Rahul Kumar Chandel and Shivani Sharma

ABSTRACT

The isolation of endophytic actinomycetes from surface sterilized parts (leave and stem) of Plumbago zeylanica (commonly known as Chitrak) was made using actinomycetes isolation agar. In the present study, two endophytic actinomycetes were isolated from root and stem and were identified as belong to Saccharopolyspora and Nocardia respectively. The in vitro antifungl activity of isolated endophytic actinomycetes has been investigated by measuring the Zone of inhibition (ZOI in mm) against the dermatophytic fungus viz. Microsporum gypseum and Microsporum canis performing different methods, as preliminary screening of actinomycetes by disk diffusion method, secondary screening by fermentation in shake flask and assay of antifungal activity from different fermented broth (GS, AGB and SCN) filtrate by well diffusion method. The two isolates of endophytic actinomycetes Saccharopolyspora and Nocardia showed significantly higher activity against both the fungus when performed by fermented broth filtrate method in case of GS and AGB which may be a good source of obtaining novel antimicrobials.

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