World Journal of
Pharmaceutical and Life Sciences

IN VITRO COMPREHENSIVE ANALYSIS OF PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTI-DIABETIC ACTIVITIES OF THREE DIFFERENT PLANTS OF BANGLADESH

Hazrat Belal, Ruksana Yesmin, Al Mamun, Nazmul Hasan, Dobirul Islam, Islam A, Tasnin MN, M. Rezaul Karim -2, Matiar Rahman, Masudul Hasan Khan, M. Ismail Hossain, Amirul Islam*

ABSTRACT

Medicinal plants are rich in phytochemicals have numerous biological activities including antioxidant, antidiabetic, anti-inflammatory, anticancer and antimicrobial activities. The present study was designed to investigate and compare the antioxidant, antidiabetic, anti-inflammatory, toxic properties and phenolic contents of methanol extracts from Syzygium cumini Bark (SCB), Bombax ceiba Fruits (BCF) and Samanea saman Flowers (SSF). Phytochemical screenings were also carried out. Among the extracts, SCB showed the highest antioxidant activity followed by BCF and SSF. Based on DPPH and Nitric oxide scavenging activity, the SCB extract was the most effective one with IC50 79.25 and 256. 3 ?g/mL. The SCB extract had the most potent inhibitory activity against lipid peroxidation with IC50 317.2 ?g/mL. In addition, the reducing capacity on ferrous ion was in the following order: SCB > BCF > SSF. The content of phenolics, flavonoids, flavonols and proanthocyanidins of SCB was found to be higher than other extracts. TPC and TFC were found strongly correlated (P<0.01) with antioxidant activities of the all plants extracts. In ?-amylase inhibition assay SCB extract causes 53.95% inhibition at concentration 1000 ?g/mL, which was significantly (P<0.05) different from another extracts. In vitro assay for anti-inflammatory activity test of BCF showed highest potency (P<0.05) in comparison to all other extracts. Alkaloids, flavonoids, phenols, carbohydrate, resins, saponins, steroids, tannins, anthracenosides and coumerins as phytochemicals were present in the extracts. The present study suggested that all the plants parts effectively ameliorate oxidative stress and thus be useful as pharmaceutical agents in various ailments. The present study also concluded that SCB have ?-amylase inhibition and BCF have anti-inflammatory potential to be used as antidiabetic and anti-inflammatory agents.

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