World Journal of
Pharmaceutical and Life Sciences

IN VITRO REGENERATION OF THREE FOREST SPECIES (DISTEPHANUS AFF GARNIERIANA; MEDINILLA MICRANTHA; MELICOPE SP. INDET) OF AMBATOVY MINE SITE (MADAGASCAR) ACCORDING TO THEIR BIOLOGICAL FORM

RAVONIARISON Nivohanintsoa Elinorovololona*

ABSTRACT

The micropropagation biotechnology was tested on the regeneration and the multiplication of three forest species of biological form different. To estimate the behavior of these species towards the technique, three parameters were studied: the axillary buds initiation, the speed of growth, and the multiplication factor. The explants with axillary buds were cultured on suitable culture medium for each species. The obtained results show the success of the in vitro micropropagation of these three species. The epiphyte lianescent species "Medinilla micrantha» showed an early of bud burst appeared to the 3rd week of culture, a speed of fast growth of 0.5cm in 17 days and a high multiplication factor of 5 in 3 months. As for the herbaceous species "Distephanus aff garnieriana ", its axillary buds began to sprout after 6th week of culture; its speed of growth is on average (0.5cm in 47 days) and 3 for its multiplication factors. Concerning the ligneous species “Melicope sp. Indet ", the starting up of axillary buds burst was more late observed after the 12th week of culture. It was a slow-growing species achieving 0.5 cm in height in 72 days and the multiplication factor was 2 within 3 months.

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