World Journal of
Pharmaceutical and Life Sciences

KINETICS OF INHIBITING EFFECT OF SOME WATER-SOLUBLE BIOANTIOXIDANTS AND THEIR MIXES IN CHEMILUMINESCENT MODEL SYSTEM

Dr. Natalya Sazhina*

ABSTRACT

Definition of antioxidant activity (AOA) of various biological objects: foodstuff, medicines, drinks, blood plasma and other human biological liquids is an important task for medical and biological researches. Now for this purpose chemiluminescent (CL) methods are widely used. They are sensitive, operate and allow controlling directly kinetics of oxidation inhibition by antioxidants (AO). Biological substrates contain a wide range of antioxidants various on the chemical structure, and the total AOA is the integrated value characterizing a possibility of their combine action taking into account potential synergism. In the present work the water-soluble CL-model of free radical oxidation of the luminal initiated by the mix "hemoglobin-hydrogen peroxide" was used. Kinetics of inhibiting action of 8 water-soluble bio antioxidants and their binary mixes has been studied. The inhibition parameters for these antioxidants and their stoichiometric coefficients were determined. Evaluation of synergetic or antagonistic interaction in mixes of used AO has made. For the majority of binary AO mixes independence of CL suppression by separate AO has been revealed and more "active" AO inhibited oxidation earlier, than less "active". The exception was only for mixes of some AO which at interaction strengthened or weakened each other, showing synergetic or antagonistic effects.

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