World Journal of
Pharmaceutical and Life Sciences

EXPRESSION AND PRODUCTION OF ENZYME LACCASE FOR POTENTIAL INDUSTRIAL APPLICATIONS

Syed Sajid Jan, Sikander Ali*, Muhammad Waqas and Noaman Yaseen

ABSTRACT

Laccases are polyphenol oxidases containing copper atoms, catalysing the oxidation of a broad spectrum of complex polyphenols and aromatic diamines compounds. They are increasingly being used in biotechnological and industrial application. In food industries laccases have been progressively more used to improve and modify the appearance, colour, and texture of various beverages and food products. Laccases have many other application in paper and textile industries and is being used frequently in bleaching and dye synthesis. To overcome its current need, higher amount of laccase availability and an adequate production systems must be developed. This review article presents new advancement about the mechanism of action of laccase catalyzing the oxidation reaction, its overexpression in various heterologous hosts such as bacteria, fungi, and yeast, various factors affecting its production, different type of fermentation processes for its overproduction, and its purification on large scale. Some of the important industrial and biotechnological application have also been discussed.

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