Abstract
METHOD DEVELOPMENT AND VALIDATION OF DRUG COMBINATION IN A FORMULATION OF ISONIAZID, RIFAMPICIN AND PIPERINE BY RP-HPLC METHOD
Merugu Manasa* and Mahesh Pothu
ABSTRACT
A simple, isocratic reverse phase high pressure liquid chromatographic method has been validation and method development of new drug combination. The method was carried out on an Waters xterra reverse phase C 18 (250 mm x 4.6 mm I.D; particle size 5 ?m) column with a mobile phase consisting of methanol and ortho phosphoric acid (pH was adjusted to 2.2 by using hydrochloric acid) in the ratio of 40:60 at a flow rate 1.0 mL/min. Detection was carried out at ? 282 nm. The retention times of Isoniazid , Rifampicin and Piperine were found to be 2.513, 3.625 and 5.316 min respectively. The developed method was validated in terms of accuracy, precision, limit of detection/quantitation, linearity and robustness. The proposed method is highly sensitive, precise, accurate and easy to perform and successfully applied for the estimation of these drugs in bulk and capsule dosage form.
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