World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

CHRONIC AMEBIASIS [ENTAMOEBA HISTOLYTICA]: DETECTION BY IMMUNODIAGNOSIS METHOD

Dr. Biswajit Batabyal*

ABSTRACT

Enzyme immunoassay (EIA) kits for Entamoeba histolytica antibody detection are commercially available. Antibody detection is most useful in patients with extra intestinal disease (amebic liver abscess) when organisms are not generally found on stool examination. A capture sandwich ELISA using purified antibodies was able to detect 70 ng of amoebae protein, showing a sensitivity of 93 % and specificity of 94%. The combination of microscopic examination and ELISA gave a concordance and discordance of 93.25% and 6.75%, respectively. It was concluded that capture sandwich ELISA is highly specific for the detection of coproantigens of E. histolytica in faeces of infected or chronic patients is quicker to perform, easier and more sensitive than microscopic examination.

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