World Journal of
Pharmaceutical and Life Sciences

BIOCHEMICAL ANALYSIS AND HOMOLOGY MODELING OF COLLAGENASE PROTEIN OF SCYLLA SERRATA CRAB

Shoba K.*, S. Sowmiya and Dr. Mazher Sultana

ABSTRACT

Scylla serrata (often called mud crab or mangrove crab, although both terms are highly ambiguous, as well as black crab) is an economically important species of crab found in the estuaries and mangroves of Africa, Australia and Asia. In their most common form, the shell colour varies from a deep, mottled green to very dark brown. The enzyme collagenase is extracted from Uca pugilator and it is estimated by using Lowry’s method. Collagenase is biochemically analysed with SDS-PAGE and HPLC. The activity of the enzyme is assayed and characterized by determining the optimum pH and temperature. The effect of activator and inhibitor on collagenase activity is obtained. To analyse the primary sequence and to predict the homology modeling of collagenase protein in Sylla serrata using bioinformatics tools Like Prot param, Swiss model server. The modeled 3D structure of the peptide clearly shows the potential antigen binding sites which would be useful in structure based drug designing studies in future.

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