World Journal of
Pharmaceutical and Life Sciences

MOLECULAR TYPING OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS STRAINS ISOLATED FROM MASTITICDAIRY COWS

Mohamed M. Hassan*, Attia A. El-Gedawy, Amena M. Ibrahim,
Alaa A. Mohamed, Ahmed Gaber

ABSTRACT

The aims of this study were to isolate and identify of methicillin resistant Staphylococcus aureus (MRSA) from mastitic cow milk. The investigation presence of mecA genes and molecular characteristics of these isolates using eight primers of repetitive element palindromic-PCR (Rep-PCR) analysis were achieved. Ninety six milk samples, collected from clinical mastitic cows from Sharkia Governorate in Egypt, were obtained from 71cowcases. MRSA isolates were recovered by pulsed field gel electrophoresis (PFGE), staphylococcal chromosomal cassette (SCC) mec typing, staphylococcal protein A. A total of 11 MRSA isolates were identified. All strains were showed a positive mecAI genes band (with size about 162 bp long) and mecAII gene band (with size about 449 bp long) and negative in mecAIII gene band (with size about of 540 bp long). The results of Rep-PCR analysis for the 11 MRSA isolates revealed approximately 114 different banding pattern, 51 of them consider as monomorphic bands with a percentage about 44.8%, and other 63 fragments consider as polymorphic bands with about 55.2%. The dendogram based on banding patterns dividing the 11 isolates into two clusters with about 67% genetic similarities. Our results indicate that the presence of sufficient nuclear DNA level variations among the studied MRSAisolates using Rep-PCR analysis, therefore, the Rep-PCR data might be a good source of information about the diversity of native S. aureus isolates.

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