World Journal of
Pharmaceutical and Life Sciences

UTILITY OF GOLD NANOPARTICLES AS QUICK, NAKED EYE COLORIMETRIC PROBE FOR DETERMINATION OF CEFPIROME SULPHATE

Abdulkareem Abdulraheem and Maha F. El-Tohamy*

ABSTRACT

The present study introduced a simple and sensitive visual and spectrophotometric method for the determination of cefpirome sulphate. Gold nanoparticles were utilized for quick detection of visual color change in the presence of cefpirome sulphate that observed by the naked eye and monitored by UV-Vis spectrophotometer. The change of color was quantitatively correlated with the quenching effect of cefpirome sulphate on the absorption band of gold nanoparticles. The experimental variables, including pH, reaction time, the effect of drug amount, the effect of the gold nanoparticles amount and temperature, etc. were investigated and optimized. Under optimal conditions the linear concentration range was found to be 0.02-120 ?g mL-1 with a correlation coefficient (r = 0.9996). The calculated lower limit of detection was 0.005 ?g mL-1 and quantification limit 0.017 ?g mL-1. The interference of some common species was investigated. The proposed method was successfully employed for quick and facile determination of the investigated drug in pure form, its pharmaceuticals and biological fluids.

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