World Journal of
Pharmaceutical and Life Sciences

EVALUATION OF SUB-ACUTE TOXICITY (ORAL) STUDY OF SIDDHA MEDICINE - SEENTHIL SARKKARAI IN WISTAR ALBINO RATS

Dr. K. S. Uma, Dr. G. Dayanand Reddy, *Dr. T. Purushoth Prabhu, Dr.S.Geethalakshmi

ABSTRACT

Seenthil sarkarai has been used by the traditional medicine practitioners for the treatment of various diseases due to its rejuvenating and tridosha samani properties. The aim of the present study was to carry out sub acute oral toxicity studies of Seenthil sarkarai in wistar albino rats. Seenthil sarkarai was prepared with the stem of Seenthil (Tinospora cordifolia) by traditional method. Twenty four wistar albino rats of either sex (3 male and 3 female) aging 6 to 7 weeks were selected based on the body weight and randomly distributed to 4 groups (Group I, II, III and IV). Distilled water was used as the vehicle and sub acute toxicity studies of Seenthil sarkarai were carried out by oral administration daily at doses of 360 mg/kg body weight, 1800 mg/kg body weight and 3600 mg / kg body weight in respective groups II- IV for 28 days. Vehicle Control group (Group-I) received distilled water only. During the study period, rats were observed weekly for toxicity symptoms, body weight gain and feed consumption. All surviving animals were euthanized on day 30th for hematological and biochemical analyses. No significant difference was observed with respect to body weight gain and feed consumption between the test groups and control group. No abnormal behavioral activity and preterminal deaths were recorded in the rats exposed to the test compound up to 10 times of the intended therapeutic dose. Thus the study demonstrates that, short term (28 days) oral intake of Seenthil sarkarai causes no toxicity upto the dose of 3600mg / kg b.wt in wistar albino rats.

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