World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

PRODUCTION AND PURIFICATION OF APHLA- HEMOLYSIN FROM PROTEUS MIRABILIS PMBS41 FROM URINARY TRACT INFECTION

Essam F. A. Al-Jumaily* and Sara Hussein Zgaer

ABSTRACT

The Proteus mirabilis pathogenic isolates (53 isolates), which isolated for urine samples from patients suffering from urinary tract infection (UTI) were found to produce hemolysin as indicated by greenish zone around colonies grown on blood agar plates. Ten isolates were screened for their production and the highest producing isolate PMBS41 which gave (42.734 Unit/ml) was selected for further experiments. Hemolysin was completely precipitated using 25-50% saturation of ammonium sulphate which gave enzyme activity (128.20unit/ml). Then followed by ion exchange chromatography using DEAE-cellulose (3.5 x 15 cm) gave only one of the many peaks showed hemolytic activity gave (48.42 unit/ml) and a yield (45.42 %), then gel filtration using Sepharose 6B column (1.5 x85 cm) gave (42.24 unit/ml) and a yield (14.90 %).

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