World Journal of
Pharmaceutical and Life Sciences

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
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Abstract

IN VITRO STUDY: INDUCED RESISTANCE OF CASSAVA (MANIHOT ESCULENTA CRANTZ.) PLANTLET AGAINST FUSARIUM OXYSPORUM BASED ON ANALYSIS OF PHENOL CONTENT

Dr. Endang Nurcahyani*, Dr. Sumardi, Dr. Bambang Irawan, Evi Yunita Sari and Tika Lidia Sari

ABSTRACT

Cassava (Manihot esculenta Crantz.) is a tropical and subtropical annual shrub from the Euphorbiaceae tribe. Tuber is widely known as a staple food-producing carbohydrates and leaves as a vegetable. Indonesia is the second largest country producing Cassava after Nigeria with an average of five years' total supply of 9.67 million tons or 10.61% of the total supply of world cassava. Central Cassava land in Indonesia is controlled by Lampung Province with cassava production in Lampung Province reaching 8.33 million tons in 2013. This situation makes Lampung a supplier of one-third of the national Cassava production from national production of 23.92 million tons. However, there are still many production constraints in Cassava cultivation, including Fusarium wilt. This disease is caused by the Fusarium oxysporum fungus, which until now still cannot be treated effectively. The use of Cassava cultivars that are resistant to fusarium wilt with high yields is expected to be an important disease control alternative. This study aims to determine and determine the specific expression character of Fo-resistant Cassava plantlets, which are phenol content. The Cassava Planlet has been initiated and selected in vitro in the Murashige and Skoog (MS) medium with the addition of fusaric acid (FA) at a concentration of 0 ppm (control), 20 ppm, 40 ppm, 60 ppm and 80 ppm. The purpose of this study was to determine the phenol content after being induced by fusaric acid and inoculated by Fusarium oxysporum. The results showed that in vitro, the concentration range of fusaric acid tolerant for selection of Cassava plantlets with optimum growth was between 20 ppm - 80 ppm; the higher the concentration of FA, the higher the phenol content of the Cassava plantlets.

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