World Journal of
Pharmaceutical and Life Sciences

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
World Journal of Pharmaceutical and Life Sciences (WJPLS) has indexed with various reputed international bodies like : Google Scholar, Index Copernicus, Indian Science Publications, SOCOLAR, China, Cosmos Impact Factor, Research Bible, Fuchu, Tokyo. JAPAN, Scientific Indexing Services (SIS), Jour Informatics (Under Process), UDLedge Science Citation Index, Global Impact Factor (In Process), International Impact Factor Services, International Scientific Indexing, UAE, International Society for Research Activity (ISRA) Journal Impact Factor (JIF), Science Library Index, Dubai, United Arab Emirates, International Innovative Journal Impact Factor (IIJIF), Scientific Journal Impact Factor (SJIF), Eurasian Scientific Journal Index (ESJI), Indian citation Index (ICI), IFSIJ Measure of Journal Quality, International Scientific Indexing, UAE (ISI), 

Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION DANAZOL IN API AND COMBINED DOSAGE FORM

Prathibha K., Jagya and Dr. A. Yasodha*

ABSTRACT

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Danazol, in its pure form as well as in tablet dosage form. Chromatography was carried out on ODS C18 (4.6 X250mm, 5?m) column using a mixture of Methonol (100% v/v) as the mobile phase at a flow rate of 1.0 ml/min, the detection was carried out at 274nm.The retention time of the Danazol was 2.252 + 0.02min.The methodproduce linear responses in the concentration range of 5-25?m/ml of Danazol. The method precision for the determination of assay was below 2.0%RSD.The method is useful in the quality control of bulk and pharmaceutical formulations.

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