World Journal of
Pharmaceutical and Life Sciences

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
World Journal of Pharmaceutical and Life Sciences (WJPLS) has indexed with various reputed international bodies like : Google Scholar, Index Copernicus, Indian Science Publications, SOCOLAR, China, Cosmos Impact Factor, Research Bible, Fuchu, Tokyo. JAPAN, Scientific Indexing Services (SIS), Jour Informatics (Under Process), UDLedge Science Citation Index, Global Impact Factor (In Process), International Impact Factor Services, International Scientific Indexing, UAE, International Society for Research Activity (ISRA) Journal Impact Factor (JIF), Science Library Index, Dubai, United Arab Emirates, International Innovative Journal Impact Factor (IIJIF), Scientific Journal Impact Factor (SJIF), Eurasian Scientific Journal Index (ESJI), Indian citation Index (ICI), 

Abstract

ANALYTICAL STABILITY INDICATING UPLC ASSAY AND VALIDATION USING C18 COLUMN FOR FLUOROMETHOLONE IN PARENTERAL DOSAGE FORM

Mohd Shafi*, Dr. Osman Ahmed and Dr. Anas Rasheed

ABSTRACT

A pecific, precise, accurate and stability indicating UPLC method is validated for estimation of Fluorometholone in parenteral inhaler dosage form. The method employed, with C18 column (250 ×4.6 mm id)—ACE Generix in gradient mode, with mobile phase of Methanol–water (62 : 38 v/v). The flow rate was 1.5 ml/min and effluent was monitored at 240nm. Retention time was found to be 5.215±0.005 min. The method was validated in terms of linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) etc. in accordance with ICH guidelines. Linear regression analysis data for the calibration plot showed that there was good linear relationship between response and concentration in the range of 20- 100?g/ml respectively. The LOD and LOQ values for were found to be 0.3245 (?g/ml) and 0.983 (?g/ml) respectively. No chromatographic interference from excipients and degradants were found. The proposed method was successfully used for estimation of Fluorometholone in parenteral dosage form.

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