World Journal of
Pharmaceutical and Life Sciences

An International Peer Reviewed Journal for Pharmaceutical and Life Sciences
An Official Publication of Society for Advance Healthcare Research (Reg. No. : 01/01/01/31674/16)
World Journal of Pharmaceutical and Life Sciences (WJPLS) has indexed with various reputed international bodies like : Google Scholar, Index Copernicus, Indian Science Publications, SOCOLAR, China, Cosmos Impact Factor, Research Bible, Fuchu, Tokyo. JAPAN, Scientific Indexing Services (SIS), Jour Informatics (Under Process), UDLedge Science Citation Index, Global Impact Factor (In Process), International Impact Factor Services, International Scientific Indexing, UAE, International Society for Research Activity (ISRA) Journal Impact Factor (JIF), Science Library Index, Dubai, United Arab Emirates, International Innovative Journal Impact Factor (IIJIF), Scientific Journal Impact Factor (SJIF), Eurasian Scientific Journal Index (ESJI), Indian citation Index (ICI), 

Abstract

DETECTION OF COUNTERFEIT STEVIA PRODUCTS USING HPLC-ELSD

Supriyadi* and Meta Kartika Untari

ABSTRACT

Diabetes prevalence in Indonesia has been increasing every year. Indonesia becomes the world's 5th largest country with diabetic population, estimated to be about 10% of the population and about 51% of those were undiagnosed. People with diabetes mellitus are required to avoid sugar (sucrose) in their daily meals and replace it with safe sugar substitutes. Stevia from Stevia rebaudiana Bertoni is known as natural sugar substitutes which safe for people with diabetes melitus. Stevia rebaudiana is also traditionally used to treat diabetes mellitus. The standard mixtures of Stevioside, Rebaudioside A, Rebaudioside C and Dulcoside A were injected into HPLC-ELSD under optimum condition to obtain the chromatograms. Samples of stevia products were extracted with methanol, and methanol extract was injected into HPLC-ELSD under the same conditions as standard solution and chromatograms were obtained. Chromatograms from stevia product samples were compared with chromatograms from Stevioside standard solutions, Rebaudioside A, Rebaudioside C and Dulcoside A. Based on the results and the above discussion it can be concluded that from the five samples, samples 1 and 2 contain stevia sweetener, sample 3 contains no stevia sweetener but rebaudioside A. Sample 4 and 5 contain neither stevia nor steviol glycoside sweeteners.

[Full Text Article]

BEST PAPER AWARDS

World Journal of Pharmaceutical and life sciences Will give best paper award in every issue in the from of money along with certificate to promote research .

Best Article of current issue
Dr. Olajide Adedayo Ajayi

Download Article : Click here